Background: A green analytical chemistry method was developed for quantification of remdesivir (CAS-1809249-37-3) in pure and pharmaceutical dosage form. The broad-spectrum antiviral drug Remdesivir (RMD) is injected intravenously. RMD has been licensed or authorized for use in treating COVID-19 in more than 50 countries during the COVID-19 epidemic. Objective: A non-destructive, sensitive, and precise attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopic technique being created and validated for the quick identification of remdesivir in pharmaceutical formulations. Methods: A method used to investigate remdesivir is obtained by measuring the carbonyl group's stretching bands within a spectral range of 1695 to 1740 cm-1. The proposed techniques were fully verified according to ICH recommendations, with linearity ranges of 1.0 to 5.0 mg g-1 for RMD.