Aim: An in-vitro quantitative evaluation of apically extruded debris during root canal instrumentation with 2 different rotary and 2 different reciprocating file systems. Materials & Method: 80 extracted human permanent mandibular single rooted premolar teeth were collected and randomly divided into four groups (n=20) based on type of files used for root canal instrumentation. Group 1: ProTaper Next and Group 2: Neoendo Flex rotary file systems, Group 3: WaveOneGold and Group 4:Reciproc Blue reciprocating file systems. Each tooth was inserted into empty eppendorf tubes till the cervical line. Distilled water was used as irrigant during canal instrumentation and apical extruded debris during instrumentation was collected into eppendorf tubes. These tubes were then incubated at 70°C for 5 days to dessicate the distilled water used. The dry apical extruded debris from each sample was weighed using Analytical Digital weighing microbalance with the accuracy of 0.0001gm. Statistical plan: One way ANOVA and Tukey’s Post-hoc tests were used.