The aim of this study is to formulate a herbal gel using extracts of Glycyrrhizaglabra, Aloe Vera, and Turmeric and evaluate its efficacy in the treatment of periodontitis. The study assesses the gel's anti-inflammatory, antimicrobial, and wound-healing properties both in vitro and in vivo. Extracts of Glycyrrhizaglabra, Aloe Vera, and Turmeric were formulated into a gel base with appropriate excipients. In vitro evaluations included antimicrobial activity against periodontal pathogens using agar diffusion methods and anti-inflammatory activity via inhibition of inflammatory markers in cell culture models. In vivo evaluations involved treating animal models (rats) of periodontitis with the herbal gel and measuring parameters such as gingival index, probing depth, and attachment loss to determine therapeutic efficacy. The results demonstrated that the herbal gel exhibited significant antimicrobial activity against periodontal pathogens, with zones of inhibition and minimum inhibitory concentrations comparable to conventional antibiotics. It also showed potent anti-inflammatory effects by suppressing the expression of pro-inflammatory cytokines. In vivo treatment with the herbal gel resulted in reduced gingival inflammation, decreased probing depth, and improved attachment gain. In conclusion, the herbal gel combining Glycyrrhizaglabra, Aloe Vera, and Turmeric shows promising potential in the treatment of periodontitis, exhibiting strong antimicrobial, anti-inflammatory, and wound-healing properties both in vitro and in vivo. Further clinical studies are warranted to validate its efficacy and safety for human use. This herbal approach offers a natural and potentially safer alternative to conventional treatment modalities for periodontitis.