The purpose of this study is to elucidate the expression of Disheveled 2 (DVL2), Axis Inhibitor 1 (AXIN1), Transcription Factor 7 (TCF7), Glycogen Synthase Kinase 3 Beta (GSK3B), in the Wnt signaling pathway, and their possible connections to triple-negative breast cancer (TNBC). The research used a cross-sectional approach to examine the expression of DVL2, AXIN1, GSK3B, and TCF7 in TNBC tissues compared to normal adjacent breast tissues in order to find biomarkers for TNBC. A total of 40 untreated patients' tumor samples were obtained after surgery and then analyzed in a laboratory equipped with molecular biology facilities. Immunohistochemistry confirmed the absence of ER, PR, HER2 receptors in TNBC. Real-Time PCR revealed significant upregulation of DVL2, AXIN1, GSK3B, and TCF7 genes in TNBC tissues, suggesting their involvement in TNBC development. These findings highlight the importance of WNT signaling pathway disruption in TNBC, particularly the role of DVL2, AXIN1, GSK3B, and TCF. The real-time PCR analysis revealed a significant upregulation of these genes in TNBC tissues relative to normal adjacent tissues, suggesting their possible involvement in the development of TNBC. In conclusion, the disruption of the Wnt signaling pathway, which involves DVL2, AXIN1, GSK3B, and TCF7, is crucial in regulating cellular processes and determining the aggressiveness of triple negative breast cancer (TNBC). Further investigation should be conducted to examine these associations and specific obstacles, since advancements in drug development focusing on Wnt proteins exhibit potential for groundbreaking therapies.