An analytical method for spectrophotometric analysis of an anticancer medication Mesna through its derivatisation as trithiocarbonate followed by complexation with Palladium (II) is described. Mesnatrithiocarbonate forms an orange-coloured 1:2 complex with Palladium (II) that has λmax 370nm. A synthesized complex created complies with Beer's law in the concentration variety of 8.351µg/ml to 1.837 X 102µg/ml. The complex is stable at neutral pH at a temperature of 30°C with correlation coefficient (r) = 0.995. Characterization of the complex includes thermal analysis, elemental analysis, FTIR, 1H NMR, ESR, and Raman spectrum studies. The complex's FTIR spectra reveal the binding of the sulphur atom of the trithiocarbonate group with palladium (II). H1 NMR studies of the complex in D2O shows further confirm the involvement of the sulphur atom of the trithiocarbonate group in complexation with Pd (II). The absence of a signal in the complex's ESR spectrum shows the diamagnetic nature of the complex. Raman spectra of the complex show the participation of the trithiocarbonate group in bonding with the central metal atom. Thermogravimetric analysis confirms the structure of the complex.