BACKGROUND: The human tissue factor plays an important role in triggering the coagulation process. It is crucial for normal embryonic growth during pregnancy. However, overexpression of this factor can pose a risk for pregnant women and the fetus, potentially leading to abortion or stillbirth. OBJECTIVE: The study aims to quantitatively assess the concentration of human tissue factor in pregnant women during various trimesters of pregnancy as well as in non-pregnant women. Additionally, the study aims to include pregnant women with a history of abortion, stillbirth, or both, to compare the results of tissue factor concentrations among these groups. PATIENTS AND METHODS: Blood samples were collected from 46 pregnant and 40 healthy non-pregnant women in gel activator tubes. Serum was obtained through centrifugation, and serum human tissue factor was measured using an enzyme-linked immunosorbent assay. RESULTS: The study revealed that there is no significant difference in human tissue factor levels among pregnant women in different age groups (P-value > 0.05). However, there is a significant difference (P-value < 0.05) between pregnant women and non-pregnant healthy females. Additionally, some pregnant women showed elevated levels of this coagulation factor, especially those who had experienced frequent abortions or stillbirths (P-value < 0.05). CONCLUSIONS: It has been found that tissue factor (TF) levels are significantly higher in pregnant women who have had an abortion or stillbirth compared to those who have not. TF can be considered as a specific and sensitive indicator of the risk of abortion in pregnant women. Therefore, measuring TF levels regularly during pregnancy and taking appropriate interventions for those with high TF levels could be beneficial for early diagnosis and prevention of abortion. Many studies support the idea that TF levels should be monitored during pregnancy to help identify and address potential risks.