Human skeleton shows a high degree of sexual dimorphism in almost every bone. Enhancing the sex accuracy from skeletal bones is one of the most important efforts in forensic anthropology. Skull and pelvic bones are considered to be the most reliable bones for identification. However, in the absence of cranium and pelvic bones, lower limb long bones play a vital role in sex determination. Present study aims to evaluate the utility of 3D volume rendering technique of CT scan for sex determination. MTL, MFL, BMB, FL, FB, FBEB, FCML, and TPML were the variables selected for investigation. The sample size was 232 (107 females and 125 males) between the age group of 18 to 80 years. Intra and inter-observer error was under the acceptable limits. The individual variable which showed the maximum sexual dimorphic character was femoral epicondyle breadth (FBEB- 89.2%), followed by bi-malleolar breadth (BMB- 87.9%). This study provides the information about the prevalent characteristics in both male and female populaces through lower limb bones measurements that will help in the results for forensic analysis. The present study is the only study conducted on lower limb bones through CT scan data for the determination of sex in Northwest Indian population according to our detailed review of literature.