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Can Combination Therapy Improve Outcomes in PDE5i-Refractory Erectile Dysfunction? A Systematic Review of Low-Intensity Extracorporeal Shockwave Therapy
Volume 8 | Issue - 8
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Influence of Storage Temperature on the Bond Performance of Universal Adhesives: A Narrative Review
Volume 8 | Issue - 8
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Effects of direct acting antivirals(DAAs)on cryoglobulinemia in patients infected with hepatitis C virus
Volume 8 | Issue - 8
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Effect of Education Sessions About Plastic Usage Hazards and Its Safe Disposal on Knowledge and Reported Practice of Female Employees at Port Said University: A Pre–Post Intervention Study
Volume 8 | Issue - 7
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Soil Quality Under a Cocoa-Based Agroforestry System With Associated Indigenous Tree Species In The Department Of Divo (Cote d’Ivoire)
Volume 8 | Issue - 7
Significance of Elevated LA1c/c Hb1peak in a HbA1c chromatogram: A delimma
Main Article Content
Abstract
Background and objective: In HbA1c estimation by HPLC method Labile A1c fraction is formed which is unstable and its concentration varies with acute changes in plasma glucose levels. Elevated peak of LA1c/Chb-1 may be due to variation in plasma glucose levels or elution of Hbf in labile A1c fraction indicating hemoglobin disorder. Methods: HbA1c is estimated in our laboratory by HPLC method on BIORAD D-10 system which is an automated cation exchange HPLC instrument. Results: Our study focused on a patient's abnormal chromatogram showing abnormal high peak of LA1c/cHb-1 along with missed Hbf in HbA1c estimation and after eliminating preanalytical error patient’s report was released with comment on possibility of hemoglobionopathy. Conclusion: Advantage of HbA1c estimation with HPLC method is to identify hemoglobinopathy can only be achieved by properly analyzing the chromatogram of each and every patient by expert technician or doctor available.
