Wheat bran is considered a widely available by-product in the wheat processing industry. It was recovered from the cereal and derivative food industry "E.R.I.A.D." in Ouled Mimoun, Algeria. This study is aimed at evaluating the physicochemical composition of wheat bran in order to assess the primary and secondary metabolites as well as the different fractions of dietary fibers. The experimental study focuses on the determination of dietary fibers in wheat bran, followed by a physicochemical analysis of the residues of primary and secondary metabolites with a cold ethanol maceration decreasing at 95%, 80%, and 65% for 24 hours. The results of the statistical study showed homogeneity of variances. Wheat bran is rich in dietary fibers, with a content of 39.5% DM, predominantly hemicelluloses (24.73%). Cellulose has a content of 10.19%, while the levels of sodium (Na) and potassium (K) are 3.87 mg/l and 3.44 mg/l, respectively, and protein content is 12.8% DM, flavonoids, and tannins. A more significant result was found to be very significant (p<0.00) during extraction with 65% ethanol, 0.265 and 0.124 mg/l (catechin equiv/100 g). Wheat bran, with its high dietary fiber content and physicochemical properties, appears to fit well within therapeutic strategies for the management of several metabolic and digestive diseases.