Snuhi kshara has been a long-established component in many of the Ayurvedic drugs. However, owing to its vigour nature, a strong validation of its pharmaceutical potency is needed. Authentication of the preparation of Snuhi kshara and the evaluation of its biocompatibility is very vital. The present study focuses on the standardization of Snuhi kshara and evaluation of its biocompatibility. Snuhi kshara was prepared following Ayurveda Sara Samgraha with appropriate modifications. Organoleptic, physicochemical parameters were recorded and biocompatibility was evaluated. It was observed that the Snuhi kshara prepared was containing phenolic compounds and exhibiting viability above 97%. The acute toxicity studies revealed that the test drug is very safe up to 2000 mg/Kg of body weight.