Background: Rice (Oryza sativa L.) is the most important staple food crop of the world. Rice is an essential source of nutrition for billions of people on the planet. there is immense need to development of high yielding coupled with quality, multiple resistance, and wider adaptive rice varieties to fulfill requirement and ensure food security. The yield is a complex trait that depends on various attributes. Therefore, the knowledge about genetic variability, Heritability and genetic advance and relationship of different attributes to yield is crucial for making efficient selection strategy. Methods: The experiment was carried out to evaluate the genetic variability, heritability and genetic advance, correlation, and path coefficient analysis in rice. The experimental material was based on a line x tester set of 24 hybrids (F1’s) developed by crossing 8 lines (females) with 3 testers (males). An attempt was made to make twenty-four cross combinations (F1’s) during Kharif season 2022. The 24 F1’s along with parents and two check varieties i.e. PR-26 and Pusa Basmati 1121 were evaluated at the Main Experimental Station of Lovely Professional University, Punjab, India in Randomized Complete Block Design with three replications during Kharif 2023.