The experiment was conducted on 20 lactating dairy cows for the 2nd-4th lactation period at 4-7 lactation months, which was carried out for three months at PT. CABS Malangbong Garut, West Java. The treatment was a complete feed with a combination of direct-fed microbials (DFM), protein bypass, Ca-PUFA, and organic mineral supplementation according to the experimental design. In vivo testing is carried out to obtain dairy cow productivity values, including milk production and milk quality, as well as hematological and biochemical testing of dairy cow blood to see an overview of livestock health based on blood conditions. Analysis of milk composition using a Lactoscan milk analyzer, while blood hematological analysis is done using an automated hematology analyzer (KT-6200 VET) and biochemical analysis of blood is done using a spectrophotometer. The research method used an experimental method with an experimental design that is a completely randomized design with five treatments with four replications. The research results were analyzed using MANOVA with further tests using DMRT α ≤ 5% in the SPSS 24 version. The results showed that the supplement combination affected milk fat and total solids content, which was apparent from the hematological and biochemical aspects profile before and after the treatment, which was relatively unaffected, thus proving there is no negative effect when given to dairy cows.