The emergence of multidrug-resistant bacteria poses a significant challenge to healthcare systems worldwide. Natural products, such as plant extracts, have garnered attention for their potential antibacterial properties. This study aimed to assess the synergistic antibacterial activity of Copper-doped Carbon Quantum Dots (Cu-CQDs) with ethanolic leaf extract of Azadirac Mission Director, Program Management Unit - National Biopharma Mission, BIRAC hta indica (A. indica) (Neem) against multidrug-resistant bacteria.The study employed standard microbiological techniques to isolate bacteria from clinical specimens and determine their antibiotic susceptibility profiles. The ethanolic leaf extract of A. indica was prepared and subjected to antibacterial susceptibility testing using disc diffusion and broth microdilution methods. Synergistic antibacterial activity of Cu-CQDs with A. indica leaf extract was demonstrated by using checkerboard assay. Results demonstrated varying degrees of susceptibility among the multidrug-resistant bacteria tested, indicating the potential of A. indica extract as an antibacterial agent. Further investigations are warranted to elucidate the mechanisms underlying its antibacterial activity and explore its potential therapeutic applications.