In this study, synthesis a series of novel triazole derivatives and evaluated their antimicrobial properties. The synthetic strategy involved the formation of triazole rings via a click chemistry approach, resulting in high yields and purity of the final products. The chemical structures of the synthesized compounds were confirmed using various spectroscopic techniques, including NMR, IR, and mass spectrometry. The antimicrobial activities of these triazole derivatives were assessed against a panel of pathogenic microorganisms, including Gram-positive bacteria, Gram-negative bacteria, and fungi, using standard antimicrobial susceptibility tests. Several of the synthesized compounds exhibited significant antimicrobial activity, with some derivatives showing potency comparable to or better than standard antibiotics. These results suggest that the novel triazole derivatives possess promising potential as antimicrobial agents and warrant further investigation for development as therapeutic agents. These synthetic compounds were present in quantities ranging from 125 μg to 500 μg/mL, they were able to successfully limit the growth of fungus by a percentage of 16-30%. It was discovered that compound 4(f) was a good antifungal agent for A. niger, but compound 4(e) displayed a moderate level of effectiveness. 4(b) shown a reduction in its activity against A. niger. Compound 4(c) was shown to be an effective antifungal agent for C. albicans, according to scientific research.