Ligands are fascinating class of ions or molecule that binds to a central metal ion to form coordination compounds. Ligand ability to donate lone pair of electrons or capacity to act as“Lewis Bases” has created tremendous wave in pharmaceutical industry. They are multifaceted class of compound formed by condensation of aldehyde or ketone with a primary amine under preliminary condition. The ligands are efficient enough to act as antibacterial, antiviral, anti-inflammatory, antiproliferative antifungal, anticancer properties. In vitro antimicrobial effect of Schiff bases encourages the researchers for the development of new anti-biofoulants products. Ligand preparation is accomplished under mild conditions with reasonably good to high yield. Organometallic complexes offer potential for the development of new materials with a wide range of applications. Due to electronic transitions which exists between Ligand and metal complexes it provides a platform for drug design that is exploited in several areas. The synthesized Alloxan 2,4 dinitrophenyl hydrazone using IR, NMR and screened for their antibacterial . The ligands are tested for their antibacterial activities on gram-positive and gram-negative bacteria.