Background : There is an association between iron status and vitamin D, suggesting an interplay between these nutrients. Vitamin D is linked to erythropoiesis and iron absorption regulation. However, evidence on its association with hemoglobin levels in pregnant women is limited and conflicting Objective Study correlation between Vitamin D and anemia in pregnant women. Methods Health clinics at Fallujah General Hospital in Anbar, Iraq, conducted a case control study involving 100 pregnant women in their first and third trimesters. The study assessed Vitamin D, serum ferritin, serum iron, and hemoglobin (HGB) using electrochemiluminescence immunoassay, cobas c311, and autoanalyzer hematology Results : In a study comparing pregnant women in the 1st trimester (n=50) and 3rd trimester (n=50), significant findings : serum vitamin D were lower in the 3rd trimester group (14.70±6.164 ng/mL) compared to the 1st trimester group (33.4±4.813 ng/mL, p = 0.016). Conversely, iron levels were higher in th e 3rd trimester(58.4±13.71 µg/dL) compared to the 1st trimester (31.5±7.85 µg/dL, p = 0.001). No correlations were found between hemoglobin and vitamin D (r = 0.039, p > 0.05), vitamin D and ferritin (r = 0.056, p > 0.05), vitamin D and iron (r = 0.187, p > 0.05). Conclusion s The vitamin D is decreased in 3ed trimester pregnant women and There is no direct correlation between Vitamin D and anemia in pr egnant women.