Background: Generally, the primary sample used for DNA testing comes from blood. Improvements in existing analytical methodologies can reduce the need for DNA levels used in examinations so that samples used can come from other parts of the body with retrieval methods that are non-invasive, more straightforward, easier to store, and more resistant to decay, for example, nails. Materials and methods: Nail clipping samples were taken from the hands and feet of an unidentified corpse, which were then divided into 5 groups: the control group without treatment, the soaking group with fresh water and salt water on days 3 and 7. DNA extraction using chloroform-phenol, measurement of DNA levels, and purity using NanoDrop spectrophotometer. Each sample representative with the lowest and highest levels was further analyzed at D18S51 loci.