Background: The chemical substances that are necessary for an organism to perform certain biological processes are named vitamins, which are crucial for metabolism. And deficiencies are more common in obese people with deficits in A, C, D, and E. Objective: This study aimed to evaluate serum concentrations of vitamin C, D (25-hydroxyvitamin D), A, and vitamin E and their association with increased body adiposity BMI in comparison to healthy subjects. Methods: 87 subjects, were divided into 2 groups: 46 obese individuals (BMI 30-39.9 kg/m2) and 41 control individuals without any multivitamin supplements (BMI 18.5-24.9 kg/m2). The study subjects underwent examination at the Ibn al-Nafis specialized laboratory (September 2019 to February 2020)., BMI was determined, and by Using the Competitive-ELISA Assay Kit, the levels of vitamins A, C, D (25-hydroxyvitamin D), and E were measured. Colorimetric Approach; Elabscience Model. SPSS 15 was used for the statistical analyses, and P-values of less than 0.05 were considered statistically significant. Results: Obese individuals' BMI and WC were considerably higher (P < 0.05) than those in healthy categories. while having significantly lower (P ≤ 0.05) levels of vitamin E and vitamin D. While the results of the study showed that obesity groups in every age group had statistically significant differences among females and males (P<0.05) and vitamin D and vitamin C when compared with healthy males and females, nonetheless, there were no notable variations in the categories of obese people of both sexes compared to healthy individuals. Furthermore, there is a significant association between vitamin C and BMI in obese individuals, but there was a notable negative association between those individuals and vitamin A, conclusion: Vitamins A, E, and D absorption issues may be correlated with obesity (higher BMI).