Nocturnal Enuresis (NE), or the condition of involuntary urination, is observed in children aged between five and sixteen worldwide, spanning all cultures, social, and economic classes. This study was conducted on 119 children in some regions within the district of Al-Shirqat in Salah al-Din province from the beginning of October 2022 to the end of January 2023, to estimate the concentration of the antidiuretic hormone (ADH). Samples were tested in the laboratory, and information pertaining to each case was recorded based on a pre-prepared form which included relevant information like name, age, and gender. The concentration of ADH in blood serum was measured using the ELISA technique