This study aimed to investigate the effects of Aspergillus flavus infection on the gastrointestinal tract (GIT) and the inflammatory response induced in animal models. Different concentrations of Aspergillus flavus were administered to animals, and various parameters such as C-reactive protein (CRP), interleukin-6 (IL-6), interleukin-8 (IL-8), and histopathological changes in the colon were assessed. The results showed that increasing doses of Aspergillus flavus led to elevated CRP, IL-6, and IL-8 levels, indicating inflammation in the GIT. However, histopathological examination did not reveal significant changes in the colon, suggesting a potential role of innate immunity in defending against fungal infections. This study provides insights into the inflammatory response in the GIT following Aspergillus flavus infection and highlights the importance of further research in this area.