Selaginella bryopteris, commonly known as 'Sanjeevani,' is a medicinal plant highly esteemed in conventional treatment because of its robust therapeutic potential. This study delves into the pharmacognostic, physicochemical, pharmacological, and formulation aspects of S. bryopteris, aiming to establish a comprehensive scientific basis for its medicinal use. Pharmacognostic evaluation involved macroscopic and microscopic analyses to characterize the plant’s morphological and anatomical features, providing crucial data for correct identification and quality control. Physicochemical studies, including determination of extractive values, moisture content, ash values, etc. were conducted to assess the purity and standardize the plant material. An analysis of the plant's phytochemical composition uncovered bioactive components that might be responsible for its many pharmacological effects, including tannins, saponins, alkaloids, flavonoids, and others. Pharmacological investigations were carried out to explore the antimicrobial properties of S. bryopteris, demonstrating significant activity that supports its traditional use in treating oxidative stress-related disorders, infections, and inflammation. Moreover, a novel formulation of S. bryopteris extract was developed, focusing on enhancing its bioavailability and therapeutic efficacy. The formulation was subjected to various physicochemical evaluations, including stability studies, to ensure its suitability for therapeutic application. This holistic approach not only reaffirms the traditional uses of S. bryopteris but also opens new avenues for its application in modern medicine. The research highlights the significance of combining traditional wisdom with evidence-based practices, providing a solid foundation for the future development of S. bryopteris-based therapeutics. The findings hold promise for further research and development of pharmaceutical formulations that harness the full potential of this remarkable plant.