A study was conducted to assess the impact of 2,4-D herbicide on mesquite trees in the field, it aimed to evaluate the possibility of using the herbicide to eradicate this invasive tree. Different rates of 2,4-D (0, 6, 12, 18, 24 grams a.i.) were dissolved in either diesel or water and applied during two consecutive seasons - winter and rainfall. It was observed that during the rainy season, the large tree treated with all rates of 2,4-D dissolved in diesel resulted in 100% mortality. During both seasons (rainy and winter), applying of 24 grams a.i. dissolved in diesel resulted in 100% mortality percentage for three tree sizes compared to the control group that used diesel only, which achieved (46.67%, 46.67% and 50.0%) and (33.33%, 33.33%, and 0.00%) for small, medium, and large sizes during the rainy and winter season, respectively. According to the study, the mortality percentage of mesquite trees varied based on the application rate of 24 grams a.i. dissolved in water (16.67%, 13.33%, 33.33%) and (0.0%, 8.33%, 8.33%) during rainy and winter seasons for small, medium, and large, respectively