Background Orthodontic tooth movement (OTM) induces biological changes within the periodontal tissues, and the analysis of gingival crevicular fluid (GCF) volume serves as a non-invasive marker to monitor these changes. This study aimed to evaluate the variation in GCF volume during early orthodontic tooth movement using clear aligners. Materials and Methods A total of 30 patients undergoing orthodontic treatment with clear aligners were recruited for the study. GCF samples were collected from the mesial and distal sites of the first molar on the right side at baseline (T0), after 7 days (T1), and after 14 days (T2) of clear aligner wear. GCF volume was measured using a Periotron 8000 device, and data were analyzed using paired t-tests to compare the GCF volume at different time points.