INTRODUCTION: Mortalities and morbidities due to Neonatal sepsis are rising in alarming numbers. The Ministryof Healthand Family welfare, India Launched “MusQan”, aninitiative topromote thequality of paediatric public health facilities. The study was done to assess of Infection control practices in Special Newborn Care Unit (SNCU), Government District hospital of Karnataka, India Materials and methods: A cross sectional study was conducted by using the MusQanassessment tool at District hospital during November 2022. Results: The assessment using the tool showed the infection control practices in the hospital are poor and not adequate. The assessment showed the following results: Prevention and measurement of Hospital acquired infection (75%), hand hygiene (56%), personal protective practices (75%), processing of equipments and instruments (70%), environmental control and physical layout (68%), biomedical waste segregation, treatment and disposal (83%). Overall, the hospital scores 71% in infection control practices. Conclusion: Neonatal sepsis is a major cause of neonatal mortality, especially in developing countries. Simple basic preventive measures like hand hygiene, adequate and regular disinfection of equipments, and use of personal protective equipments among healthcare personnel can reduce the incidence and prevalence of neonatal sepsis.