Background: UTIs are among the most prevalent hospital-acquired infections (HAIs) and are frequently brought on by members of the Enterobacteriaceae family. The appearance of Extended - Spectrum Beta - Lactamase (ESBL) – producing strains is a grave danger to antimicrobial therapy and has led to restricted antimicrobial options and to high morbidity. Aim: The purpose of this study was to determine the frequency and pattern of ESBL producing Enterobacteriaceae that were isolated from hospitalized patients' urine samples, as well as to evaluate their pattern of antibiotic susceptibility. Methods: The isolates came from 899 samples of urine collected at SGT Hospital, Gurugram, during a six-month period and yielded 109 non- duplicate Enterobacteriaceae “isolates. Identification was performed using standard biochemical assays of the isolates. In accordance with the standards established by the Clinical and Laboratory Standards Institute (CLSI), the Kirby-Bauer disk diffusion method (mKB) has been utilised to conduct the test for antimicrobial susceptibility. blaSHV, blaCTX-M, and blaTEM were discovered by multiplex PCR in twenty randomly selected ESBL-positive isolates. The Double Disk Synergy Test (DDST) was utilised in order to screen and validate the production” of ESBL. Results: Of the 899 urine samples, 12.1% (109/899) were significantly positive for the presence of enterobacteriaceae. “Escherichia coli (83.4%) and Klebsiella pneumoniae (6.4%) were the predominant isolates” found. It was determined that 51 isolates (46.8%) produced ESBL by phenotypic screening, of which E.coli accounted for 88.2% of ESBL producers. A high level of resistance was noted for Amoxicillin (76.1%), ampicillin (69.7%), and cefepime (68.8%), while fosfomycin (89.0% sensitive) and aztreonam (87% sensitive) were highly effective. The blaSHV gene was found in 95% of the 20 isolates, blaCTX -M in 40%, and blaTEM was not found, based on PCR analysis. Conclusion: According to the study, blaSHV-carrying E. coli is primarily responsible for the high incidence of ESBL-producing Enterobacteriaceae (46.8%) in UTI isolates from hospitalized patients. Controlling the spread of these multidrug-resistant bacteria requires constant antimicrobial surveillance, appropriate antimicrobial use, and the application of infection control strategies.