Aim: A study to assess the effectiveness of educational programme on knowledge, perception and acceptability regarding cervical cancer among adolescents in Bhiwani, Haryana. Materials and Methods: This study was designed as a pre-post interventional study to assess the effectiveness of an educational program on knowledge, perception, and acceptability regarding cervical cancer among adolescents. A total of 100 adolescents, aged 15-19 years, were recruited from various high schools and community centers in the region. The inclusion criteria were adolescents who provided informed consent and were available for the entire duration of the study. Exclusion criteria included adolescents with a prior diagnosis of cervical cancer or those who had already received extensive education on cervical cancer. Results: Knowledge levels about cervical cancer significantly improved post-intervention. Before the educational program, 30% of participants had no knowledge about cervical cancer, which dropped to 5% after the intervention (p<0.01). Similarly, basic knowledge decreased from 50% to 20%, while moderate and advanced knowledge levels increased from 15% to 50% and from 5% to 25%, respectively (all p<0.01). These results indicate the effectiveness of the educational program in enhancing adolescents' knowledge about cervical cancer.A substantial majority (90%) of participants agreed or strongly agreed that cervical cancer education is important, compared to 70% pre-intervention (p<0.01). The willingness to participate in screening programs increased to 85%, up from 60% pre-intervention (p<0.01). Belief in the effectiveness of HPV vaccination also rose to 85% post-intervention from 65% pre-intervention (p<0.01). These positive changes highlight the impact of the educational program on adolescents' perceptions. Knowledge improvements were significant across all demographic variables. Both genders showed marked increases in knowledge, with males increasing from a mean score of 2.5 to 3.5 (p<0.01) and females from 2.3 to 3.6 (p<0.01). Age groups also exhibited significant knowledge gains: those aged 15-16 improved from 2.2 to 3.4 (p<0.01), those aged 17-18 from 2.4 to 3.5 (p<0.01), and those aged 19 from 2.6 to 3.6 (p<0.01). These results demonstrate the broad effectiveness of the educational program. Conclusion: In conclusion, the educational program significantly improved knowledge, perceptions, and acceptability regarding cervical cancer among adolescents. These findings are consistent with other studies that highlight the effectiveness of targeted educational interventions in healthcare.