Tamus communis L. is commonly used as medicinal plants in Algeria against a variety of human disease. In this study, the extraction of rhizomesof T. communisby methanol gave a dry matter yield of 6.45%. The effects of T. communis methanolic extract (TCME) were examined per os on female rats Albino Wistar for six weeks. Determination of serum parameters biochemi cal, haematological and evaluation of physical and histopathology of organs of treated rats were studied. The study of acute toxicity showed a low toxicity with LD50 > 12000 mg / kg of body weight of rats treated with TCME. These data can be used to classify these two plants in low toxicity and / or slightly toxic. However, the subacute treatment of rats with the doses of 75, 150 and 300 mg of TCME / kg body weight of rats for six weeks resulted in signifi cant increases of haematological parameters: RBC, MCV, RDW, HCT, PLT, MPV, MCH and HGB. Biochemical analysis revealed significant increase of renal. On the other hand, TCME did not cause significant changes biochemi cal parameters; but a significant decrease (p ≤ 0.05) of relative mass of ova ries in rats treated. Histopathological examination has not marqued modifica tions of organs structure.