The genus Mentha L. (Lamiaceae) is distributed all over the world and can be found in many environments. The study evaluated the acute toxicity of the aqueous extract from the leaves of Mentha suaveolens in Swiss mice. The mice were administered single oral doses of 2000 mg/kg and 5000 mg/kg and were monitored for death and body weight for the first four hours, as well as over a period of 24 hours and at least once daily for 14 days. The results indicated that the LD50 (lethal dose at which 50% of the population dies) was found to be greater than 5000 mg/kg, suggesting that the plant extract is relatively safe. Furthermore, there were no significant variations in body and organ weights between the control group and the group treated with the extract after 24 days of treatment. Biochemical analysis of the mice showed no differences in the examined parameters. Additionally, histological examination did not reveal any changes in the organs of the treated mice compared to the control group. These findings indicate that the aqueous extract demonstrated drug safety in mice. These results suggest that the aqueous extract of Mentha suaveolens is safe for use in mice.