Urinary tract infections (UTIs) are among the most common bacterial infections, often complicated by the rising issue of antibiotic resistance, which limits treatment options and poses significant public health challenges. This study aimed to evaluate the antibiotic resistance and sensitivity profiles of the most common uropathogens in the University Health Centre, Tarnaka and Astra Hospital, Hyderabad, Telangana, South India. A total of 80 urine samples were collected from UTI patients, and the isolates were tested for resistance to five antibiotics: Amoxicillin, Gentamicin, Norfloxacin, Ofloxacin, and Streptomycin, using the Kirby-Bauer disc diffusion method. The antibiotic sensitivity and resistance profile of bacterial pathogens revealed varied responses. E. coli showed high sensitivity to Gentamicin, Ofloxacin, and Streptomycin, with 35 isolates sensitive to each, while 12 were resistant to Amoxicillin. Klebsiella demonstrated sensitivity primarily to Gentamicin (15) and Ofloxacin (15) but exhibited resistance to Amoxicillin (11). Proteus and Pseudomonas showed low sensitivity and resistance overall, with Proteus having minimal resistance to Amoxicillin (2) and Pseudomonas exhibiting moderate sensitivity to Gentamicin (8). These findings highlight the potential efficacy of Gentamicin and Ofloxacin against multiple pathogens and underscore the importance of antibiotic susceptibility testing to guide treatment strategies.