Pasteurella multocida is responsible for infectious diseases across various animal species. It causes fowl cholera in birds and acts as a secondary contributor to respiratory syndromes. Antimicrobial therapy remains an effective treatment option. Generally, isolates of P. multocida are responsive to most commonly used medicinal antibiotics; however, their overuse and misuse result in the development of resistant strains. We characterized the antimicrobial sensitivity of 89 P. multocida strains obtained from poultry against 14 widely used antibiotics— ampicillin, colistin, azithromycin, enrofloxacin, erythromycin, florfenicol, flumequine, neomycin, fosphomycin, penicillin, trimethoprim + sulfamethoxazole, tetracycline, streptomycin, and gentamicin—using the disk diffusion technique. Most of the strains tested were vulnerable to most antimicrobial agents. Nevertheless, notable resistance was observed against ampicillin, making these compound unsuitable for treating infections caused by P. multocida. Conversely, the antimicrobial effectiveness of classical florfenicle, gentamycin, and fosphomycin were identified as effective agents for this bacterium. Fowl cholera has led to substantial economic losses in numerous poultry-producing nations around the world. In Iraq, outbreaks of fowl cholera are often reported across various types of poultry production. The antimicrobial susceptibility testing revealed several multidrug-resistant strains among the P. multocida isolates. All isolates were high resistant to penicillin (50%), streptomycin (13%), tetracycline (10%), enrofloxacin (6%), florfenicol (11%), Erythromycin (14%), gentamicin (16%), and amoxicillin (14%). This study offers valuable epidemiological insights into P. multocida and its role in fowl cholera outbreaks in broilers in the central region of Iraq, particularly in Babylon