Hepatocellular carcinoma (HCC) is a primary malignant liver tumor with a general prognosis of death. This encourages the search for anticancer candidates against HCC cytotoxicity using natural ingredients such as plants. The water guava plant (Syzygium aqueum) is a medicinal plant that is widely grown in tropical areas. This study aims to determine the anticancer activity of ethyl acetate extract of Syzygium aqueum leaves by investigating its impact on Huh7it cell proliferation. The leaves of the water guava plant (Syzygium aqueum) were extracted using ethyl acetate. Anticancer activity was examined via MTT (3-(4,5- dimethylthiazol-2yl)-5-(3 carboxymethoxy phenyl)-2-(4- sulfophenyl)-2H tetrazolium) on Huh7it liver cancer cells. The MTT test results showed that water guava leaves (Syzygium aqueum) had an IC50 value of 163.3 μg/ml.