Background: The aim of the present study was to evaluate the impact of human dental pulp stem cell-conditioned medium (DPSC-CM) on apoptosis, proliferation, and angiogenesis in tongue squamous cell carcinoma cells. Methods: Dental pulp stem cells were derived from sound-extracted third molars and cultured under optimal conditions. After the isolated cells were characterized, the conditioned culture media were collected. HNO97 cells were treated with DPSC-CM for 24 hours or 48 hours and then compared to the control group. Analysis of cell apoptosis and proliferation was conducted using flow cytometry to measure the expression of Annexin and ki67. Cell cycle progression was studied, and the expression of VEGF was analyzed using RT‒PCR to assess angiogenesis. Results: The number of apoptotic cells increased, with the greatest increase observed in HNO97 cells treated with DPSC-CM for 48 hours. Cell necrosis decreased as the treatment period increased. Flow cytometric analysis of ki67 expression demonstrated a reduction in the quantity of actively dividing cells. HNO97 cells were arrested at the G1/S phase. Rt-PCR evaluation of VEGF indicated a reduction in angiogenesis in HNO97 cells. Conclusion: DPSC CM inhibited the proliferation and angiogenesis of HNO-97 cells, which became more pronounced as the treatment duration increased.