The current study aimed to determine the phenolic contents and antioxidant properties of methanol (Met. E) and aqueous (Aq. E) extracts of Asperula hirsuta. Met. E showed the highest levels of polyphenols (144.68 ± 10.87 µg GAE/mg extract) and flavonoids (32.78 ± 2.06 µg QE/mg extract). Both extracts exhibited potent scavenging activity with IC50 values of 19.54 ± 1.60 μg/mL and 42.14 ± 0.57 μg/mL, respectively. At 250 µg/mL, Aq. E exerted stronger chelating effect (97%) than Met. E (86%). However, Met. E antioxidant capacity (169.01 ± 13.25 μg AAE/mg extract) was more than that of Aq. E (125.84 ± 07.12 μg AAE/mg extract). Both extracts protected from β-carotene bleaching by 61% and 66%, respectively. The findings of this study suggest that Asperula hirsuta extracts may serve as a promising source of natural antioxidants.