Objectives: To assess knowledge and recognition of sepsis among healthcare professionals in medical unit. METHODS: This was a cross-sectional study conducted in Divisional Headquater Teaching Hospital, Mirpur Azad Kashmir from June 2023 to February 2024. The International Sepsis Survey questionnaire was adapted, and it was distributed to trainee physicians as well as specialists, and consultants practicing in various hospitals as hard copies and collected back after the response from the health professional. Knowledge and perception were scored and 50% was considered the cut-off score for adequacy. Data was analyzed using SPSS version 22. RESULTS: The survey gathered responses from 176 participants, revealing that 45.7% of them demonstrated sufficient knowledge on the subject. Regarding knowledge about the sepsis, Statistical analysis revealed a significant association between the specialty, the duration of working experience, position and age of the responding medical professionals (p < 0.05), whereas there was no statistically significant difference in gander regarding the knowledge about the sepsis (p> 0.05). Regarding the recognition of sepsis there was statistically significance only in duration of the medical practice and the specialty (p < 0.05), while recognition of sepsis was not statistically significant in duration of medical practice, age and gender of the participants (P> 0.05). A significant majority of respondents, accounting for 76.14%, reported a lack of awareness regarding the qSOFA (quick Sequential Organ Failure Assessment) scoring system, indicating it remains an unmet need in this field. CONCLUSION: Sepsis stands as one of the most commonly misdiagnosed conditions in critical care settings, underscoring a pressing necessity for its early and accurate diagnosis. Furthermore, the timely initiation of appropriate management protocols for suspected sepsis is deemed imperative to enhance patient outcomes