In this present investigation, the amino acids and keratin proteins rich in chicken feather waste and fish wastes were hydrolysed into organic liquid fertilizer by bio- hydrolysation using B. licheniformis and B. subtilis. With the optimized bioprocess parameters such as 10 ml inoculum volume, incubation temperature at 37 ℃, agitation rate at 175 rpm for the incubation of degradation medium at 8.5 pH value with 12.5 g of chicken feathers separately, 12.5 g of fresh solid fish separately and 12.5 g of combined waste of chicken feathers and fresh solid fish waste separately, bio-hydrolysation was established. Incubated flasks were observed at the different time intervals of the 1st day, 4th day, 8th day, and 12 th day and samples were collected after the 12 th day. Corresponding collected samples were filtered and centrifuged. The supernatants obtained were analysed to determine nitrogen content as 7500 mg/L, 4240 mg/L, and 6104 mg/L, phosphorous content as 1295 mg/L, 2100 mg/L, and 1850 mg/L, potassium content as 4710 mg/L, 5608 mg/L, and 5300 mg/L, total amino acid content as 4.38%, 2.15%, and 3.96% and total antioxidant capacity as 190 µg/ml, 240 µg/ml and 228 µg/ml, respectively. Thus, the liquid fertilizer was produced by an eco- friendly approach through microbial degradation.