The term "molecular markers" refers to certain DNA sequences that are connected to genes or phenotypes that researchers are interested in examining. There is a greater likelihood that these sequences are connected to certain genes due to the fact that they are readily detectable. In order to fulfill their function as chromosomal genetic landmarks, these markers contribute to the identification of certain regions. The dicotyledonous plants that are classified under the genus Brassica are all members of the same family, which is known as the Brassicaceae . A reduced degree of evergreenness may be seen in them. Plants that belong to the cruciferae family are easily identifiable by the cross-shaped flowers that they produce. Genetic variation, we are talking to the changes that occur within the genes that make up an individual of a species. These changes may be found in the constitutions of heredity. For the sake of preserving the developmental stability and biological potential of plant species, this variety is absolutely necessary. The detection of a wide range of polymorphism and genetic distance brought to light the presence of a huge range of variety across Brassica species. This diversity occurred throughout a wide range of genetic distances. The variants of BS-10 and BS-14 have the largest amount of genetic variety with regard to the variations that were used in this study.