Background- Clostridium difficile infection (CDI) represents a major clinical challenge in hospital settings, associated with increased morbidity and healthcare costs. Methods: This retrospective study was conducted on 180 hospitalized patients diagnosed with CDI, analyzing data from January 2020 to December 2022 at a tertiary care center. The study assessed epidemiological patterns, clinical characteristics, and outcomes of CDI. Results: The prevalence of CDI was higher in patients over 65 years of age and those receiving antibiotic therapy. Complications included severe diarrhea, colitis, and in some cases, death. The study also found a significant length of stay increase and higher readmission rates among patients with CDI. Conclusion: CDI significantly burdens hospitalized patients, highlighting the need for stringent infection control practices and targeted therapeutic strategies. This study underscores the importance of early diagnosis and management to improve clinical outcomes.