The study investigates the impact of varying grazing intensities on the biochemical composition of Artemisia diffusa, a key species in rangeland ecosystems. Grazing, an essential ecological process, can significantly influence plant biochemistry, affecting both nutritional value and ecological interactions. This research was conducted in a semi-arid Karnabchul desert rangelands, where A. diffusa samples were collected from plots subjected to different grazing intensities: (low grazing and heavy grazing). Biochemical analyses focused on key constituents such as crude protein, fiber, carbohydrates including. The results indicated significant variations in the biochemical composition of A. diffusa across different grazing intensities. Light grazing led to a moderate increase in protein and carbohydrate contents, enhancing the plant's nutritional value. In contrast, heavy grazing caused a reduction in these protein and carbohydrate but significantly not change the concentrations of fiber, likely as a defensive response to grazing pressure.