Indonesia is the largest coffee producer in the world. Kalosi coffee is one of the arabica coffees grown in Enrekang Regency. Fermentation of coffee beans aims to improve the taste of coffee and release the mucus layer attached to the coffee beans. The stages of this research are coffee beans are prepared for the roasting process to produce a medium roast maturity level. Next, grinding is carried out to obtain a fine grind size. Next, ground coffee was extracted, and the brew was filtered using a fine sieve (100 mesh), after which the filtrate was put into a freeze dryer. After drying, instant coffee was weighed for the next process, namely the addition of palm sugar according to the treatment (A1 = 25%, A2 = 35%, and A3 = 45%). Organoleptic and chemical tests were then conducted. The best formulation of Kalosi Arabica instant coffee with added palm sugar is a formulation of 65% instant coffee and 35% added palm sugar. The conclusion is that the best formulation of instant coffee contains antioxidants that are classified as strong at 39 ppm, caffeine at 0.76%, and pH 4.90. The addition of palm sugar affects the chemical content of coffee, such as stronger antioxidants and decreased caffeine.