The present study investigated the antioxidant, anticancer, and anti-neurodegenerative potential of Rudraksha alkaloid rich extract. The antioxidant activity of RARE was measured by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 2'-azino-bis3-ethylbenzthiazoline-6-sulfonic acid (ABTS) free radical scavenging activities while anti-cancer and, anti-neurodegenerative properties was evaluated through MTT assay, and acetylcholinesterase enzyme inhibition activities respectively. The study outcomes demonstrated a comparable dose-dependent response of RARE with that of reference standards for the tested biomedical properties. The IC50 values for antioxidant, anti-cancer, and anti-neurodegenerative activities were obtained. Further, in vivo safety and efficacy assessments are warranted to confirm their in vitro biomedical applications.