Coagulase-Negative staphylococci (CoNS) are increasingly recognized as important opportunistic pathogens in health- care settings. These organisms are commonly found on human skin and mucous membranes but can cause serious infections, particularly in immunocompromised patients and those with implanted medical devices. The aim of this study is to investigate the prevalence and distribution of CoNS species in various hospital environment sites and assess their potential role as reservoirs for health-care associated infections. This study was conducted in Algerian hospital over 6 months. 200 environmental samples were collected from different sites (beds, door, tables, serum stands, etc.). isolates were identified API Staph kits and Vitek 2 t automated system. Antibiotic susceptibility was determined by disk diffusion method. A total of 78 Staphylococcus strains (52 CoNS strains) were recovered from 200 samples. the most prevalent species identified were Staphylococcus haemolyticus (n=15), Staphylococcus hominis (n=10) and Staphylococcus Epidermidis (n=7). Higher concentrations were found on frequently touched surfaces. High levels of antibiotic resistance were observed, particularly against Penicillin (96%). Notably, all strains demonstrated the ability to form biofilms. Our findings highlight the ubiquitous presence of CoNS in hospital environment. The high prevalence of antibiotic-resistant strains underscores the importance of effective infection control measures and judicious use of antibiotics to mitigate the risk of healthcare-associated infections caused by these opportunistic pathogens