Background: In recent years, the research is mainly focusing on Enterococcus faecalis on the various antibiotics. Therefore, greater attention has been paid to antimicrobial activity screening and evaluating methods. Materials and Methods:The zone of inhibition was determined by agar well diffusion method varied for different antibiotic (1.amoxicillin, 2.amoxicillin-Potassium Clavulanate 3.Doxycycline – Lactobacillus 4.Ampicillin - Dicloxacillin, 5.Tinidazole-Norfloxacin-Lactobacillus, 6.Cefuroxime, 7.Ciprofloxacin, 8.Ofloxacin, 9.Cefixime, 10.Azithromycin) assays. Each antibiotic was placed in MHA plates with different concentrations of 30 microlitres, 60 microlitres, and 90 microlitres of respective diluted antibiotics. Results: The results obtained in the agar diffusion plates were in fairly every bacteria as action against E. faecalis. But at a low concentration of 30 microlitres, ofloxacin has the most zone of inhibition and least is cefuroxime. At a concentration of 60 microliter concentration and in that ciprofloxacin shows maximum activity and cefuroxime shows minimum activity. At the concentration of 90 microliter concentration and in that ciprofloxacin shows maximum activity and cefixime shows minimum activity. Conclusion:It was concluded that at lower level concentration, ofloxacin had higher efficacy than ciprofloxacin in inhibiting the bacterial growth against E. faecalis, which is the predominant organism in the failure of root canal treatment.