Objective- To compare the amount of debris extruded by Hand K file, Neoendo Flex, Protaper Next, XP Endo Shaper files. Materials and methods- 100 human extracted mandibular premolar with single canal similar length of 15mm were grouped as hand-K File(n=25), Neo Endo Flex(n=25), Protaper Next(n=25) and XP endo Shaper(n=25). Separate Eppendorf tube were used for each sample to collect debris. Pre weight of effendorf tube were taken. After instrumentation, eppendorf tubes were incubated at 680 C for 5 days. Post-weight of Eppendorf tubes were taken again . The weight of debris was calculated by subtracting pre weight from post-weight. Statistical Analysis used- results obtained were tabulated and statistical analysis was performed using the statistical package SPSS 26.0 (SPSS Inc., Chicago, IL). Apical extrusion of the four different root canal preparation systems was performed by using One-way ANOVA analysis. A Post-hoc Boneferroni test was used for multiple comparisons. The level of significance was set at P<0.05. Results- Maximum amount of apical debris extrusion was seen in Group I (K-file) followed by Group II (Neoendo Flex), Group IV (XP), Group III (Protaper Next). Conclusion- All the instrument used in this study showed apical debris extrusion.