Background: Dengue fever, an environmentally-based disease transmitted by Aedes mosquitoes, remains a health problem in tropical and subtropical countries, with 143,000 cases in Indonesia by the end of 2022. Objective: This study aimed to analyze the differences of DHF determinants in endemic and non-endemic areas of Makassar City and Maros Regency. Methodology: The type of research used is comparative analytic with cross sectional design. The population in this study was the entire number of households involving 222 samples taken by Proportional Random Sampling, and the hypothesis test used was the Mann whitney test. Results: Bivariate statistical analysis showed that there were significant differences in the density of larvae (p=0.000), mosquito breeding sites (p=0.000), the number of free larvae (p=0.024), attitudes (p=0.001), hanging clothes (p=0.009), installing mosquito screens (p=0.006) between endemic and non-endemic areas. However, there was no significant difference in the level of knowledge (p=0.450), motivation (p=0.347) between the two areas. Conclusion: This study showed significant differences in larval density, mosquito breeding sites, larval free counts, behaviors, and measures of hanging clothes and installing wire mesh between endemic and non-endemic areas.