Background: Nerve conduction studies are valuable for evaluating peripheral nervous system disorders, but the impact of age, gender, and BMI on nerve conduction velocity (NCV) remains inadequately understood. Objectives: The objective was to identify gender-related NCV disparities within different BMI categories and investigate any age-related associations. Methodology: It was a cross-sectional carried out at the Department of Physiology, Baqai Medical University in Karachi, from January 2017 to July 2017 It involved 500 participants aged 18-45, equally split between genders, from the local population. Nerve conduction velocity (NCV) was recorded using a Power Lab after stimulating the ulnar and peroneal nerves at the wrist and the lateral aspect of the knee, respectively. NCV assessments were conducted for ulnar sensory, ulnar motor, and peroneal motor nerves. Results: Gender-based disparities in Nerve Conduction Velocity (NCV) are prominent in different BMI categories, with males consistently exhibiting significantly higher NCV than females across all BMI groups (p < 0.001). While age shows no significant correlations with NCV, BMI is positively related to ulnar sensory NCV (p < 0.05) and negatively associated with peroneal motor NCV (p < 0.05). Conclusion: In conclusion, this study highlights the significant influence of gender on Nerve Conduction Velocity across different BMI categories, emphasizing the limited impact of age.