The treatment of apical periodontitis has long been a crucial responsibility in the field of endodontics. Non-hermetic root canal filling has been implicated in the failure, despite studies indicating a 91% success rate. Sealing ability and bioactivity are interesting qualities that enhance the results of root canal treatment. The goal of root canal treatment is to both prevent and cure apical periodontitis. Following institutional review board permission, this experimental investigation was carried out in the Dental Material Science Department at AIMDC from March 2023 to January 2024. The computed total sample size was 28, which was rounded to 30. The Thirty extracted single rooted human teeth were collected, disinfected, and stored in saline. The teeth were decoronated to obtain 12 mm standardized root segments. Statistical analysis revealed a significant difference (p < 0.05) between the bioactive glass sealer and the commercially available sealers, with the novel sealer demonstrating the highest bond strength.