Citrus is the most important genera of the Citrus family, having been cultivated round the globe and familiar for its nutritional, dietary, and therapeutic value for human health. This plant has enormous amounts of phytochemicals in all the parts including roots, shoots, leaves, fruits, and fruit peels. Citrus maxima are the largest fruit variety of citrus family and well known for its various ethnomedicinal significance and long been employed to treat various illnesses. It gained popularity due to its rich phytochemicals with medicinal significances, however only a few studies have been conducted in exploring the phytochemicals of their leaves. Hence, we hypothesis that the leaves of the Citrus maxima may be equally possessing a lot of phytochemicals which of clinical significance as like the earlier reported spectrum in its fruits and fruit peels. The main objective of our study focuses on analyzing the chemical compounds present in the leaves of the Citrus maxima, identifying the secondary metabolites and their biological activity. The leaf extract was thus obtained using methanol and water as solvents. The phytochemical compounds like phenols, saponins, terpenoids, flavonoids, steroids, quinines, steroids and sugar were present in the extracts. Total phenol, DPPH, FRAP, SOD and total antioxidant activity were found to be very significant. Further, the presence of various compounds in the leaf extracts were identified using UV Visible, FTIR, TLC and GC-MS. The plant extracts possessed antimicrobial and antifungal properties against common human pathogen. This study proves that the leaf extract holds potential antioxidant, biological and pharmacological significances.