To overcome from this problem and production of quality fruits, this study was conducted to optimize organic manure and inorganic fertilizers with the aid of bio-fertilizers on growth, yield characters and flowering performance characteristics of fruits. The experiment was conducted in a Randomized Block Design (RBD) with three replications having ten treatments with different doses of NPK, FYM, Vermicompost, Azotobactor and PSB either single or in combination during two (2022–23 & 2023–24) consecutive in Rabi seasons. The results showed that application of T5 –( PSB + 75% P + 100% N&K) significantly improved the growth, yield characters and flowering performance of fruits (plant height, number of branches, days to first flowering, days to 50% flowering, days to 1st fruiting number of flowers clusture per plant, number of flowers per clusture, number of fruits per plant, days to harvesting, fruit length, fruit diameter, fruit yield kg/plant, fruit yield kg/plot and fruits yield q/ha. ) with soil application during both the years (2022–23 & 2023–24). The findings from our two-year study on tomatoes cultivated in sandy loam soils provide a solid scientific foundation for the use of bio-fertilizers and organic materials, promoting cleaner food production and improved environmental conditions and also helps farmers for more fruit production per ha yield for higher profitability.