Background & Methods: The aim of the study is to study Cost-effectiveness of Antibiotics prescribed for UTIs in Non-Pregnant females visiting in OBG & Gynaecology. Clinical analyses of their urine, urino-genital system were carried out as per standard protocol. The urine culture confirming urinary tract infection positive cases were taken as infected population for further analysis. The case history age group and other demographic factors of the positive cases were recorded. Results: Total 200 patients were treated with the different drugs. Most of the patients (30%) were treated with the Sulfamethoxazole. Nitrofurantoin prescribe in (25.4%) patients. Flavoxate prescribe in (17.6%) patients. Gentamycin prescribe in (14.70%) patients and (10.7%) patients treated with Tetracyclin. Conclusion:UTI is a serious public health problem if untreated. Early diagnosis and prompt treatment will prevent the chances of developing further complication of UTI and will help to reduce the sufferings of the patient, hospital stay and economic loss.We observed that the cost effective and effective treatment in the UTI infection is affordable with the Nitrofurantoin treatment.