This study was designed to production of zinc oxide nanoparticles, and to determining of minimum inhipetory concentration(MIC) against p. aeruginosa isolates The green method was used in manufacturing zinc oxide nanoparticles with clove extract; 0.5 g zinc chloride was added to 10 ml clove extract, then incubated with a shaker overnight at 25 ºC. The supernatants were centrifuged at 8000 rpm for 20 m. zinc oxide nanoparticle products were characterised using qualitative and quantitative tests. Morphological observation of the supernatants colour change was made after adding zinc chloride colour change into dark brown and light brown, respectively, was the first indicator. The colour of the supernatant was changed. The optimum condition for ZnO biosynthesis was characterised through several techniques such as UV-Vis, AFM, EDX, FT-IR, and FE-SEM. In particular, a cut-off phenomenon of the green synthesised ZnO was found at around 280 nm using UV-Vis, while spherical shape particles were noticed using FE-SEM techniques. Also, the AFM analysis revealed that ZnO NPs have an average diameter size of 45.02 nm.The green synthesized ZnO NPs exhibited a minimum inhibition concentration (3.125 µg/ml) against P. aeruginosa isolates. These results suggest that green synthesized ZnO NPs have a strong inhibitory effect on P. aeruginosa isolates.